|
| 產(chǎn)地 | 中國(guó) |
| 品牌 | solarbio |
| 貨號(hào) | G1120 |
| 用途 | 蘇木精-伊紅染色法 ( Hematoxylin-Eosin staining )，簡(jiǎn)稱HE染色法 ，切片技術(shù)里常用的染色法之一 |
| 包裝規(guī)格 | 3×100ml |
| CAS編號(hào) | |
| 純度 | 現(xiàn)詢客服% |
| 是否進(jìn)口 |
貨號(hào) | 產(chǎn)品 | 規(guī)格 | 價(jià)格 |
G1120 | HE 染色液 ( 試劑盒 ) | 3 × 10ml | 140.00 |
G1120 | HE 染色液 ( 試劑盒 ) | 3 × 100ml | 400.00 |
產(chǎn)品說(shuō)明：
蘇木精-伊紅染色法 ( Hematoxylin-Eosin staining )，簡(jiǎn)稱 HE 染色法 ，是病理學(xué)常規(guī)制片中最基本的染色方法。蘇木精染液為堿性 ，主要使細(xì)胞核內(nèi)的染色質(zhì)與胞質(zhì)內(nèi)的核糖體著紫藍(lán)色；伊紅為酸性染料，主要使細(xì)胞質(zhì)和細(xì)胞外基質(zhì)中的成分著紅色 。染色過(guò)程需要優(yōu)化，著色情況與組織或細(xì)胞的種類有關(guān)，也隨其生活周期及病理變化而改變。例如，很多細(xì)胞在新生時(shí)期胞漿對(duì)伊紅著色較淡或輕度嗜堿，當(dāng)其衰老時(shí)或發(fā)生退行性變則呈現(xiàn)嗜伊紅濃染。膠原纖維在老化和出現(xiàn)透明變性時(shí)，伊紅著色由淺變深。本產(chǎn)品所包含試劑均為工作液，可直接使用。
操作說(shuō)明（以下步驟僅供參考）：
（一）石蠟組織切片染色。
1．取材組織塊，經(jīng)固定后，常規(guī)石蠟包埋，切片。
2．石蠟切片脫蠟水化：
①二甲苯（I）中脫蠟 5min。
②換用新鮮的二甲苯（Ⅱ），再脫蠟 5min。
③無(wú)水乙醇 5 min。
④95%乙醇 2min。
⑤80％乙醇 2min
⑥70%乙醇 2min。
⑦蒸餾水 2min。
3．蘇木素染液染色 5-20min(具體時(shí)間根據(jù)染色結(jié)果和實(shí)驗(yàn)要求調(diào)整)，自來(lái)水沖洗。
4．分化液分化 30s。
產(chǎn)品內(nèi)容 G1120-10 G1120-100
蘇木素染液 10ml 100ml
分化液 10ml 100ml
伊紅染液 10ml 100ml
5．自來(lái)水浸泡 15min 或溫水（約 50℃）5min。
6．置伊紅染液幾秒-2min(具體時(shí)間根據(jù)染色結(jié)果和實(shí)驗(yàn)要求調(diào)整)，自來(lái)水沖洗。
7．自來(lái)水浸泡 2-5min。
8．脫水，透明，封片：
①95％乙醇（I）2-3s
②95％乙醇（Ⅱ）2-3s
③100％乙醇（I）2-3s
④100％乙醇（Ⅱ）1min
⑤二甲苯石炭酸（3：1）1min
⑥二甲苯（I）1min
⑦二甲苯（Ⅱ）1min
⑧中性樹(shù)膠封固，鏡下觀察。
（二）冰凍切片
冰凍切片不用脫蠟，可固定后直接染色，其方法與石蠟切片相同。
注意事項(xiàng)：
1、切片脫蠟應(yīng)盡量干凈。
2、系列乙醇應(yīng)經(jīng)常更換新液。
3、第一次使用本試劑盒時(shí)建議先取 1-2 個(gè)樣品做預(yù)實(shí)驗(yàn)。
4、染色過(guò)程推薦淺染，通常只需能夠分辨細(xì)胞核即可，顏色過(guò)深有可能影響細(xì)胞質(zhì)顏色。
5、冷凍切片染色時(shí)間盡量要短。
6、 為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
相關(guān)產(chǎn)品：
G1010 姬姆薩染色液
G1040 瑞氏染色液
G1100 伊紅染色液
G1140 蘇木素染色液（常規(guī)染色）
P2100 10×多聚賴氨酸
產(chǎn)品說(shuō)明：
蘇木精 - 伊紅染色法 ( Hematoxylin-Eosin staining ) ，簡(jiǎn)稱 HE 染色法 ，切片技術(shù)里常用的染色法之一 。蘇木精染液為堿性 ，主要使細(xì)胞核內(nèi)的染色質(zhì)與胞質(zhì)內(nèi)的核糖體著紫藍(lán)色；伊紅為酸性染料，主要使細(xì)胞質(zhì)和細(xì)胞外基質(zhì)中的成分著紅色 。
染色過(guò)程需要優(yōu)化，著色情況與組織或細(xì)胞的種類有關(guān)，也隨其生活周期及病理變化而改變。例如，很多細(xì)胞在新生時(shí)期胞漿對(duì)伊紅著色較淡或輕度嗜堿，當(dāng)其衰老時(shí)或發(fā)生退行性變則呈現(xiàn)嗜伊紅濃染。膠原纖維在老化和出現(xiàn)透明變性時(shí)，伊紅著色由淺變深。
G1020 瑞氏-姬姆薩復(fù)合染液 100ml/500ml 220/800
G1040 瑞氏染色液 50ml/100ml 100/160
G1060 革蘭氏染色液(試劑盒) 4×(10/100/500)ml 80/400/1200
G1061 結(jié)晶紫染色液（2.5%） 10/100/500ml 40/120/400
G1062 結(jié)晶紫染色液（1%） 10/100/500ml 40/120/400
G1063 結(jié)晶紫染色液（0.1%） 10/100/500ml 40/120/400
G1066 盧戈碘液（革蘭氏染色） 10/100/500ml 40/120/400
G1067 番紅染色液（沙黃） 10/100/500ml 40/120/400
G1069 盧戈碘液（標(biāo)準(zhǔn)碘液） 100ml/500ml 120/400
G1080 Mayer'蘇木素染液(免疫組化) 10ml/100ml/500ml 60/320/1400
G1100 伊紅染色液 100ml/500ml 160/480
G1120 HE染色液(試劑盒) 3×10ml/3×100ml 140/400
G1130 莢膜染色液（試劑盒） 2*50/2*100/2*250ml 160/260/520
G1132 芽孢染色液（試劑盒） 2*50/2*100/2*250ml 160/260/520
G1134 鞭毛染色液（試劑盒） 100ml 240
G1142 Weigert蘇木素染色液 2*50/2*100/2*500ml 140/210/620
G1140 Cole氏蘇木素染色液(常規(guī)染色) 100ml/500ml 210/620
G1160 石炭酸復(fù)紅染液(苯酚品紅染液) 100ml/500ml 160/500
G1165 改良型石炭酸復(fù)紅染液(苯酚品紅染液) 100ml/500ml 200/800
G1170 抗酸染色液（試劑盒） 3*50/3*100/3*250ml 200/300/600
G1180 0.1%呂氏堿性美藍(lán)染色液 100ml/500ml 160/500
G1185 0.4%呂氏堿性美藍(lán)染色液 100ml/500ml 160/500
G1330 Van Gieson 染色液(VG染色液) 50/100/500ml 60/100/300
G1333 Van Gieson 染色液(VG染色液，含蘇木素) 3*50/3*100/3*500ml 180/300/900
G1210 溴甲酚綠-甲基紅指示劑溶液 100ml/500ml 100/260
G1220 甲苯胺藍(lán)溶液 100ml/500ml 140/400
G1260 飽和油紅O染色液 100ml/500ml 100/260
G1270 亮綠SF染色液（1%） 100ml/500ml 120/400
G1310 中性紅染色液(0.33% 過(guò)濾除菌，活細(xì)胞染色) 100ml/500ml 200/600
G1315 中性紅染色液(0.5% 細(xì)胞或組織切片染色) 100ml/500ml 160/500
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